Production of thermostable extracellular α-amylase by a moderate thermophilic Bacillus licheniformis isolated from Qinarje Hot Spring (Ardebil prov. of Iran)

Thermophilic Bacillus Licheniformis Rbs 5 Isolated from Hot Tunisian Spring Co-producing Alkaline and Thermostable Α-amylase and Protease Enzymes

Production of a thermostable extracellular amylase from thermophilic Bacillus species

Isolation and identification of thermophilic Bacillus sp was carried out from a soil sample. The cells were cultivated in a medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the medium shortened the lag period and improved the growth and amylase synthesis. The optimum temperature for amylase production was detecte...

Production of a Thermostable Extracellular Protease from Thermophilic Bacillus Species

Isolation and identification of thermophilic Bacillus sp was carried out from soil samples of a tannery area. The isolate KCPSS-3, when cultivated in liquid cultures containing trisodium citrate, produced protease. The production reached a maximum in 9 h, with levels of 2.15 U/mg protein. The microorganism utilized several carbon sources for the production of protease. Starch was found to be th...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

BACKGROUND Some strains of Bacillus licheniformis have been improved by target-directed screening as well as by classical genetic manipulation and used in commercial thermostable alpha-amylase and alkaline protease production for over 40 years. Further improvements in production of these enzymes are desirable. RESULTS A new strain of B. licheniformis CBBD302 carrying a recombinant plasmid pHY...